pAAV/D377Y-mPCSK9
(Plasmid #58376)

• Purpose: Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis
• Depositing Lab: Jacob Bentzon
• Publication: Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. doi: 10.1161/CIRCRESAHA.114.302937. Epub 2014 Mar 27.

Backbone

• Vector backbone: pAAV/mU6.siRNA.shuttle
• Backbone manufacturer: Applied Viromics, Fremont, CA, USA
• Total vector size (bp): 6519
• Modifications to backbone: mU6.siRNA cassette removed
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: proprotein convertase subtilisin/kexin type 9
• Alt name: PCSK9
• Alt name: NARC1
• Species: M. musculus (mouse)
• Insert Size (bp): 2085
• Mutation: D377Y
• Entrez Gene: Pcsk9 (a.k.a. AI415265, AI747682, FH3, HCHOLA3, Narc1, PC9)
• Promoter: HCRApoE/hAAT

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: FseI (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Obtained from RT-PCR of mouse liver
• Articles Citing this Plasmid:
  • 25 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry