pCMV-SNAP-CRY2-VHH(GFP)
(Plasmid #58370)

• Purpose: For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP
• Depositing Lab: Won Do Heo
• Publication: Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 6486
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2[1-498]
• Alt name: cryptochrome 2
• Alt name: AT-PHH1
• Alt name: ATCRY2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1494
• GenBank ID: 839529
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • SNAP (N terminal on insert)
  • vhhGFP4 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry