Skip to main content
Addgene

pCMV-CRY2(D387A)-mCherry
(Plasmid #58369)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 6243
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2[1-498] (D387A)
  • Alt name
    cryptochrome 2
  • Alt name
    AT-PHH1
  • Alt name
    ATCRY2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1494
  • Mutation
    D387A
  • GenBank ID
    839529
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-CRY2(D387A)-mCherry was a gift from Won Do Heo (Addgene plasmid # 58369 ; http://n2t.net/addgene:58369 ; RRID:Addgene_58369)

  • For your References section:

    Reversible protein inactivation by optogenetic trapping in cells. Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD. Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4. 10.1038/nmeth.2940 PubMed 24793453
Related items:
Commonly requested with: