pCMV-CIB1-mCerulean-MP
(Plasmid #58366)

• Purpose: For use in light-induced protein inactivation through the interaction with CRY2
• Depositing Lab: Won Do Heo
• Publication: Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4.

Backbone

• Vector backbone: pmCerulean-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4742
• Total vector size (bp): 5720
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CIB1[1-147]
• Alt name: cryptochrome-interacting basic-helix-loop-helix 1
• Alt name: T4L20.110
• Alt name: T4L20_110
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 513
• GenBank ID: 829604
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCerulean (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV Forward

Gene/Insert 2

• Gene/Insert name: Camk2a[315-478]
• Alt name: calcium/calmodulin-dependent protein kinase II alpha
• Alt name: PKCCD
• Alt name: PK2CDD
• Species: R. norvegicus (rat)
• Insert Size (bp): 498
• GenBank ID: 25400
• Entrez Gene: Camk2a (a.k.a. PK2CDD, PKCCD)
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry