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Purpose(Empty Backbone) Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58352 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDDGFP2_LEU2D
- Backbone size (bp) 8693
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Vector typeYeast Expression
- Promoter GAL1
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Selectable markersLEU2, URA3
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Tag
/ Fusion Protein
- GFP-His (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDDGFP_LEU2D was a gift from Simon Newstead (Addgene plasmid # 58352 ; http://n2t.net/addgene:58352 ; RRID:Addgene_58352) -
For your References section:
Method to increase the yield of eukaryotic membrane protein expression in Saccharomyces cerevisiae for structural and functional studies. Parker JL, Newstead S. Protein Sci. 2014 Jun 20. doi: 10.1002/pro.2507. 10.1002/pro.2507 PubMed 24947543
