pEGFP-NAD
(Plasmid #58345)

• Purpose: pEGFP-Mirt with a short intron from NDUFS1 cloned in
• Depositing Lab: Matthew Wood
• Publication: Seow et al RNA. 2012 Jul;18(7):1328-37. doi: 10.1261/rna.030601.111. Epub 2012 May 30.

Backbone

• Vector backbone: peGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Site for intron addition added into the middle of the eGFP ORF. BspEI, BglII, XhoI, SacI sites removed from the MCS
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NDUSF1 intron 6
• Species: H. sapiens (human)
• Insert Size (bp): 91
• Entrez Gene: NDUFS1 (a.k.a. CI-75Kd, CI-75k, MC1DN5, PRO1304)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (not destroyed)
• 3′ cloning site: BbsI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40 poly A Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry