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Addgene

pcDNA3.1-hNox1
(Plasmid #58344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NADPH oxidase 1
  • Alt name
    Nox1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • GenBank ID
    27035
  • Entrez Gene
    NOX1 (a.k.a. GP91-2, MOX1, NOH-1, NOH-1L, NOH1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-hNox1 was a gift from Botond Banfi & Karl-Heinz Krause (Addgene plasmid # 58344 ; http://n2t.net/addgene:58344 ; RRID:Addgene_58344)

  • For your References section:

    A mammalian H+ channel generated through alternative splicing of the NADPH oxidase homolog NOH-1. Banfi B, Maturana A, Jaconi S, Arnaudeau S, Laforge T, Sinha B, Ligeti E, Demaurex N, Krause KH. Science. 2000 Jan 7;287(5450):138-42. 8151 [pii] PubMed 10615049
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