pcDNA3.1-hNox3
(Plasmid #58341)

• Purpose: expresses human Nox3 in mammalian cells
• Depositing Lab: Botond Banfi
• Publication: Kiss et al Curr Biol. 2006 Jan 24;16(2):208-13.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NADPH oxidase 3
• Alt name: Nox3
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Entrez Gene: NOX3 (a.k.a. GP91-3, MOX-2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry