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Addgene

pcDNA3.1-mNoxa1
(Plasmid #58339)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58339 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NADPH oxidase activator 1
  • Alt name
    Noxa1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1335
  • Entrez Gene
    Noxa1 (a.k.a. NY-CO-31, SDCCAG31)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-mNoxa1 was a gift from Botond Banfi & Karl-Heinz Krause (Addgene plasmid # 58339 ; http://n2t.net/addgene:58339 ; RRID:Addgene_58339)
  • For your References section:

    Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. Banfi B, Clark RA, Steger K, Krause KH. J Biol Chem. 2003 Feb 7;278(6):3510-3. Epub 2002 Dec 6. 10.1074/jbc.C200613200 PubMed 12473664