pcDNA3.1-mNoxa1
(Plasmid
#58339)
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Purposeexpresses mouse Noxa1 in mammalian cells
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 6800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNADPH oxidase activator 1
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Alt nameNoxa1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1335
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Entrez GeneNoxa1 (a.k.a. NY-CO-31, SDCCAG31)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-mNoxa1 was a gift from Botond Banfi & Karl-Heinz Krause (Addgene plasmid # 58339 ; http://n2t.net/addgene:58339 ; RRID:Addgene_58339) -
For your References section:
Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. Banfi B, Clark RA, Steger K, Krause KH. J Biol Chem. 2003 Feb 7;278(6):3510-3. Epub 2002 Dec 6. 10.1074/jbc.C200613200 PubMed 12473664