pcDNA3.1-mNox3
(Plasmid #58337)

• Purpose: expresses mouse Nox3 in mammalian cells
• Depositing Labs: Botond Banfi, Karl-Heinz Krause
• Publication: Banfi et al J Biol Chem. 2004 Oct 29;279(44):46065-72. Epub 2004 Aug 23.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NADPH oxidase 3
• Alt name: Nox3
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Entrez Gene: Nox3 (a.k.a. GP91-3, het, nmf250)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry