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Addgene

S142A drebrin-YFP
(Plasmid #58335)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    drebrin
  • Alt name
    drebrin E isoform
  • Species
    H. sapiens (human)
  • Mutation
    S142A, phospho-dead mutant
  • Entrez Gene
    DBN1 (a.k.a. D0S117E)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    S142A drebrin-YFP was a gift from Phillip Gordon-Weeks (Addgene plasmid # 58335 ; http://n2t.net/addgene:58335 ; RRID:Addgene_58335)

  • For your References section:

    Drebrin contains a cryptic F-actin-bundling activity regulated by Cdk5 phosphorylation. Worth DC, Daly CN, Geraldo S, Oozeer F, Gordon-Weeks PR. J Cell Biol. 2013 Sep 2;202(5):793-806. doi: 10.1083/jcb.201303005. Epub 2013 Aug 26. 10.1083/jcb.201303005 PubMed 23979715
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