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pDDGFP2_leu2d_NRT1.1
(Plasmid #58332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58332 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDDGFP2_LEU2D
  • Backbone size w/o insert (bp) 8700
  • Total vector size (bp) 10500
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2, URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRT1.1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1800
  • Entrez Gene
    NRT1.1 (a.k.a. AT1G12110, ARABIDOPSIS THALIANA NITRATE TRANSPORTER 1, ATNRT1, ATNRT1.1, AtNPF6.3, B-1, CHL1, CHL1-1, CHLORATE/NITRATE TRANSPORTER, CHLORINA 1, F12F1.1, F12F1_1, NITRATE TRANSPORTER 1, NITRATE/CHLORATE TRANSPORTER, NPF6.3, NRT1, NRT1/ PTR family 6.3, nitrate transporter 1.1)
  • Promoter GAL1
  • Tag / Fusion Protein
    • GFP-His (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CTATACTTTAACGTCAAGG
  • 3′ sequencing primer CAGTGAATAATTCTTCACC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDDGFP2_leu2d_NRT1.1 was a gift from Simon Newstead (Addgene plasmid # 58332 ; http://n2t.net/addgene:58332 ; RRID:Addgene_58332)

  • For your References section:

    Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1. Parker JL, Newstead S. Nature. 2014 Mar 6;507(7490):68-72. doi: 10.1038/nature13116. Epub 2014 Feb 26. 10.1038/nature13116 PubMed 24572366
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