pEGFP-Mirt
(Plasmid
#58330)
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Purpose(Empty Backbone) Allows the insertion of an intron into eGFP via BbsI sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepeGFP-C1
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Backbone manufacturerClontech
- Backbone size (bp) 4700
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Modifications to backboneSite for intron addition added into the middle of the eGFP ORF. BspEI, BglII, XhoI, SacI sites removed from the MCS
- Promoter CMV
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-Mirt was a gift from Matthew Wood (Addgene plasmid # 58330 ; http://n2t.net/addgene:58330 ; RRID:Addgene_58330) -
For your References section:
Artificial mirtron-mediated gene knockdown: functional DMPK silencing in mammalian cells. Seow Y, Sibley CR, Wood MJ. RNA. 2012 Jul;18(7):1328-37. doi: 10.1261/rna.030601.111. Epub 2012 May 30. 10.1261/rna.030601.111 PubMed 22647847
