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pCMV6-Entry IL36RN R48W
(Plasmid #58324)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58324 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6-entry
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 5300
  • Vector type
    Entry vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IL36RN
  • Species
    H. sapiens (human)
  • Mutation
    R48W: c.142C>T, p.Arg48Trp
  • GenBank ID
    NM_012275.2
  • Entrez Gene
    IL36RN (a.k.a. FIL1, FIL1(DELTA), FIL1D, IL-36Ra, IL1F5, IL1HY1, IL1L1, IL1RP3, IL36RA, PSORP, PSORS14)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc-DDK (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SgfI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-Entry IL36RN R48W was a gift from Francesca Capon (Addgene plasmid # 58324 ; http://n2t.net/addgene:58324 ; RRID:Addgene_58324)

  • For your References section:

    In Silico and In Vitro Analysis of IL36RN Alterations Reveals Critical Residues for the Function of the Interleukin-36 Receptor Complex. Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F. J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 10.1016/j.jid.2023.06.191. Epub 2023 Jul 4. 10.1016/j.jid.2023.06.191 PubMed 37414245
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