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pEGFP-TRAF4(K190R)
(Plasmid #58319)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6112
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TNF receptor associated factor 4
  • Alt name
    TRAF4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1412
  • Mutation
    K 190 to R
  • GenBank ID
    NM_009423 BC020455
  • Entrez Gene
    Traf4 (a.k.a. A530032M13Rik, CART1, msp2)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mouse TRAF4 cDNA was obtained from cDNA clone IMAGE 3598556

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-TRAF4(K190R) was a gift from Ying Zhang (Addgene plasmid # 58319 ; http://n2t.net/addgene:58319 ; RRID:Addgene_58319)

  • For your References section:

    Ubiquitination of tumor necrosis factor receptor-associated factor 4 (TRAF4) by Smad ubiquitination regulatory factor 1 (Smurf1) regulates motility of breast epithelial and cancer cells. Wang X, Jin C, Tang Y, Tang LY, Zhang YE. J Biol Chem. 2013 Jul 26;288(30):21784-92. doi: 10.1074/jbc.M113.472704. Epub 2013 Jun 11. 10.1074/jbc.M113.472704 PubMed 23760265
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