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Addgene

pRK-HA-TRAF5
(Plasmid #58315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Genentech
  • Backbone size w/o insert (bp) 4754
  • Total vector size (bp) 6430
  • Modifications to backbone
    HA tag is inserted before ClaI site
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNF receptor associated factor 5
  • Alt name
    TRAF5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1676
  • GenBank ID
    NM_011633 BC012702
  • Entrez Gene
    Traf5
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer RK5R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mouse TRAF5 cDNA was obtained from cDNA clone MGC-13966

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK-HA-TRAF5 was a gift from Ying Zhang (Addgene plasmid # 58315 ; http://n2t.net/addgene:58315 ; RRID:Addgene_58315)

  • For your References section:

    Ubiquitination of tumor necrosis factor receptor-associated factor 4 (TRAF4) by Smad ubiquitination regulatory factor 1 (Smurf1) regulates motility of breast epithelial and cancer cells. Wang X, Jin C, Tang Y, Tang LY, Zhang YE. J Biol Chem. 2013 Jul 26;288(30):21784-92. doi: 10.1074/jbc.M113.472704. Epub 2013 Jun 11. 10.1074/jbc.M113.472704 PubMed 23760265
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