pSEVAT1K1A
(Plasmid #58298)

• Purpose: (Empty Backbone) GeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as cargo.
• Depositing Lab: Tom Ellis
• Publication: Wright et al ACS Synth Biol. 2014 May 13.

Backbone

• Vector backbone: SEVA
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. coli MC1061 DIAL EI ΔthyA-Kis
• Growth instructions: For plasmid propagation, a host with an integrated GeneGuard genomic cassette is required.
• Copy number: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Kid/Kis. Toxin-Antitoxin parts. Part source: Prof. Ramoń Diáz-Orejas (Dept of Molecular Microbiology and Infection Biology, Centro de Investigaciones Bioloǵicas, Madrid);DIAL strains. Part source: Prof J. Christopher Anderson (Dept of Bioengineering, University of California, Berkeley);COR parts. Part source: Prof Vićtor de Lorenzo (Systems Biology Program, Centro Nacional de Biotecnologiá, Madrid).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.