Skip to main content
Addgene

pcDNA3.1 myc-RNF114
(Plasmid #58295)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1(-) Myc/His
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNF114
  • Alt name
    ring finger protein 114
  • Species
    H. sapiens (human)
  • Entrez Gene
    RNF114 (a.k.a. PSORS12, ZNF313)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • his (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 myc-RNF114 was a gift from Francesca Capon (Addgene plasmid # 58295 ; http://n2t.net/addgene:58295 ; RRID:Addgene_58295)

  • For your References section:

    Functional analysis of the RNF114 psoriasis susceptibility gene implicates innate immune responses to double-stranded RNA in disease pathogenesis. Bijlmakers MJ, Kanneganti SK, Barker JN, Trembath RC, Capon F. Hum Mol Genet. 2011 Aug 15;20(16):3129-37. doi: 10.1093/hmg/ddr215. Epub 2011 May 13. 10.1093/hmg/ddr215 PubMed 21571784
Related items:
Commonly requested with: