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Addgene

pcDNA3.1-myc-AP1S3 R33W
(Plasmid #58294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP1S3
  • Alt name
    adaptor-related protein complex 1, sigma 3 subunit
  • Species
    H. sapiens (human)
  • Mutation
    R33W: c.97C>T,p.Arg33Trp
  • GenBank ID
    NM_001039569.1
  • Entrez Gene
    AP1S3 (a.k.a. PSORS15, sigma1C)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-myc-AP1S3 R33W was a gift from Francesca Capon (Addgene plasmid # 58294 ; http://n2t.net/addgene:58294 ; RRID:Addgene_58294)

  • For your References section:

    AP1S3 mutations are associated with pustular psoriasis and impaired Toll-like receptor 3 trafficking. Setta-Kaffetzi N, Simpson MA, Navarini AA, Patel VM, Lu HC, Allen MH, Duckworth M, Bachelez H, Burden AD, Choon SE, Griffiths CE, Kirby B, Kolios A, Seyger MM, Prins C, Smahi A, Trembath RC, Fraternali F, Smith CH, Barker JN, Capon F. Am J Hum Genet. 2014 May 1;94(5):790-7. doi: 10.1016/j.ajhg.2014.04.005. 10.1016/j.ajhg.2014.04.005 PubMed 24791904
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