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Addgene

pERB174
(Plasmid #58291)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58291 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    inducible knockdown/cDNA RMCE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Mis12-GFP-FKBPx3
  • Alt name
    pERB174
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • GFP

Gene/Insert 2

  • Gene/Insert name
    inducible FRB-mCh
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    FKBP-targeted miRNA

Gene/Insert 4

  • Gene/Insert name
    FRB-mCh-Mad1^549-718
  • Tag / Fusion Protein
    • mCherry

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pERB174 was a gift from Michael Lampson (Addgene plasmid # 58291 ; http://n2t.net/addgene:58291 ; RRID:Addgene_58291)

  • For your References section:

    Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint. Ballister ER, Riegman M, Lampson MA. J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113. 10.1083/jcb.201311113 PubMed 24637323
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