pERB172
(Plasmid
#58290)
-
Purposeconst. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-718-AAA
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 58290 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneinducible knockdown/cDNA RMCE
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameMis12-GFP-FKBPx3
-
Alt namepERB172
-
SpeciesH. sapiens (human)
-
Tag
/ Fusion Protein
- GFP
Gene/Insert 2
-
Gene/Insert nameinducible FRB-mCh
-
SpeciesH. sapiens (human)
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Gene/Insert 3
-
Gene/Insert nameFKBP-targeted miRNA
Gene/Insert 4
-
Gene/Insert nameFRB-mCh-Mad1^485-718-AAA
-
Tag
/ Fusion Protein
- mCherry
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pERB172 was a gift from Michael Lampson (Addgene plasmid # 58290 ; http://n2t.net/addgene:58290 ; RRID:Addgene_58290) -
For your References section:
Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint. Ballister ER, Riegman M, Lampson MA. J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113. 10.1083/jcb.201311113 PubMed 24637323