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Addgene

pRSETC PITPbeta N89F
(Plasmid #58279)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58279 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET-C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PITP beta
  • Alt name
    phosphatidylinositol transfer protein
  • Species
    H. sapiens (human)
  • Mutation
    N89F (abolishes PI binding)
  • Entrez Gene
    PITPNB (a.k.a. PI-TP-beta, PtdInsTP, VIB1B)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC sequencing has found some mutations in the insert, which should not effect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETC PITPbeta N89F was a gift from Shamshad Cockcroft (Addgene plasmid # 58279 ; http://n2t.net/addgene:58279 ; RRID:Addgene_58279)

  • For your References section:

    Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum. Carvou N, Holic R, Li M, Futter C, Skippen A, Cockcroft S. J Cell Sci. 2010 Apr 15;123(Pt 8):1262-73. doi: 10.1242/jcs.061986. Epub 2010 Mar 23. 10.1242/jcs.061986 PubMed 20332109
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