pcDNA3.1 myc-PITPalpha
(Plasmid
#58277)
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Purposemammalian expression of human PITP alpha
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58277 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePITP alpha
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Alt namephosphatidylinositol transfer protein, alpha
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SpeciesH. sapiens (human)
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Entrez GenePITPNA (a.k.a. HEL-S-36, PI-TPalpha, PITPN, VIB1A)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC sequencing has identified some discrepancies in the insert, they should not effect the function of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 myc-PITPalpha was a gift from Shamshad Cockcroft (Addgene plasmid # 58277 ; http://n2t.net/addgene:58277 ; RRID:Addgene_58277)