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pRSETC-RdgBbeta-splice variant 1
(Plasmid #58276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET-C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RdgBbeta
  • Alt name
    phosphatidylinositol transfer protein, cytoplasmic 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PITPNC1 (a.k.a. M-RDGB-beta, MRDGBbeta, RDGB-BETA, RDGBB, RDGBB1)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The RdgBbeta-splice variant 1 was originally obtained from Fullwood et al J Biol Chem. vol 274., p31553-31558 and matches that sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETC-RdgBbeta-splice variant 1 was a gift from Shamshad Cockcroft (Addgene plasmid # 58276 ; http://n2t.net/addgene:58276 ; RRID:Addgene_58276)

  • For your References section:

    The phosphatidylinositol transfer protein RdgBbeta binds 14-3-3 via its unstructured C-terminus, whereas its lipid-binding domain interacts with the integral membrane protein ATRAP (angiotensin II type I receptor-associated protein). Garner K, Li M, Ugwuanya N, Cockcroft S. Biochem J. 2011 Oct 1;439(1):97-111. doi: 10.1042/BJ20110649. 10.1042/BJ20110649 PubMed 21728994
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