pcDNA3.1 Flag RdgBbeta (long form) splice variant 1
(Plasmid
#58275)
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Purposemammalian expression of human RdgBbeta splice variant 1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1 (-)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRdgBbeta
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Alt namephosphatidylinositol transfer protein, cytoplasmic 1
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SpeciesH. sapiens (human)
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Entrez GenePITPNC1 (a.k.a. M-RDGB-beta, MRDGBbeta, RDGB-BETA, RDGBB, RDGBB1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 Flag RdgBbeta (long form) splice variant 1 was a gift from Shamshad Cockcroft (Addgene plasmid # 58275 ; http://n2t.net/addgene:58275 ; RRID:Addgene_58275) -
For your References section:
The phosphatidylinositol transfer protein RdgBbeta binds 14-3-3 via its unstructured C-terminus, whereas its lipid-binding domain interacts with the integral membrane protein ATRAP (angiotensin II type I receptor-associated protein). Garner K, Li M, Ugwuanya N, Cockcroft S. Biochem J. 2011 Oct 1;439(1):97-111. doi: 10.1042/BJ20110649. 10.1042/BJ20110649 PubMed 21728994