PITPbeta-GFP
(Plasmid
#58274)
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Purposemammalian expression of GFP-PITP beta
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58274 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePITP beta
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Alt namephosphatidylinositol transfer protein, beta, splice variant 1
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SpeciesH. sapiens (human)
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Entrez GenePITPNB (a.k.a. PI-TP-beta, PtdInsTP, VIB1B)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PITPbeta-GFP was a gift from Shamshad Cockcroft (Addgene plasmid # 58274 ; http://n2t.net/addgene:58274 ; RRID:Addgene_58274) -
For your References section:
Differential expression of a C-terminal splice variant of phosphatidylinositol transfer protein beta lacking the constitutive-phosphorylated Ser262 that localizes to the Golgi compartment. Morgan CP, Allen-Baume V, Radulovic M, Li M, Skippen A, Cockcroft S. Biochem J. 2006 Sep 15;398(3):411-21. 10.1042/BJ20060420 PubMed 16780419