pRSETC-PITPbeta (splice variant 1)
(Plasmid
#58270)
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Purposebacterial expression of PITP beta (phosphatidylinositol transfer protein beta)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58270 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSET-C
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePITP beta
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Alt namephosphatidylinositol transfer protein
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SpeciesH. sapiens (human)
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Entrez GenePITPNB (a.k.a. PI-TP-beta, PtdInsTP, VIB1B)
- Promoter T7
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Tag
/ Fusion Protein
- HIS (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETC-PITPbeta (splice variant 1) was a gift from Shamshad Cockcroft (Addgene plasmid # 58270 ; http://n2t.net/addgene:58270 ; RRID:Addgene_58270) -
For your References section:
Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells. Shadan S, Holic R, Carvou N, Ee P, Li M, Murray-Rust J, Cockcroft S. Traffic. 2008 Sep;9(10):1743-56. doi: 10.1111/j.1600-0854.2008.00794.x. Epub 2008 Jul 9. 10.1111/j.1600-0854.2008.00794.x PubMed 18636990