pCAG-HA-rat Nrxn3beta AS4(-)
(Plasmid #58269)

• Purpose: Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)
• Depositing Lab: Peter Scheiffele
• Publication: Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4868
• Total vector size (bp): 6221
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nrxn3beta AS4(-)
• Alt name: Neurexin 3 beta AS4(-)
• Alt name: Nrxn3beta Delta4
• Alt name: Nrxn3
• Species: R. norvegicus (rat)
• Insert Size (bp): 1353
• Entrez Gene: Nrxn3
• Promoter: CAG
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')

Resource Information

• Supplemental Documents:
  • pCAG-HA-rat Nrxn3beta AS4(-)_sequence file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI).
Insert can also be released by single digest using XhoI.
The rat neurexin 3 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4).

Note: HA-epitope tag was introduced at the mature N-terminus.