pCAG-HA-rat Nrxn1beta AS4(-)
(Plasmid #58267)

• Purpose: Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)
• Depositing Lab: Peter Scheiffele
• Publication: Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4869
• Total vector size (bp): 6216
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nrxn1beta AS4(-)
• Alt name: Neurexin 1-beta
• Species: R. norvegicus (rat)
• Insert Size (bp): 1347
• Mutation: see comments
• GenBank ID: M96375
• Entrez Gene: Nrxn1 (a.k.a. Nrxn1b)
• Promoter: CAG
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')

Resource Information

• Supplemental Documents:
  • pCAG-HA-rat Nrxn1beta AS4(-)_sequence file
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI).
Insert can also be released by single digest using XhoI.
The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).

Note: HA-epitope tag was introduced at the mature N-terminus.