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Addgene

pBabe-FLAG-Neuroserpin
(Plasmid #58261)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58261 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-Puro
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 6810
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Neuroserpin
  • Alt name
    SERPINI1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1605
  • GenBank ID
    BC018043.1
  • Entrez Gene
    SERPINI1 (a.k.a. HNS-S1, HNS-S2, PI12, neuroserpin)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer gtctctcccccttgaacctc
  • 3′ sequencing primer ATGGCTTCCTCAGGGAAAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-FLAG-Neuroserpin was a gift from Joan Massague (Addgene plasmid # 58261 ; http://n2t.net/addgene:58261 ; RRID:Addgene_58261)

  • For your References section:

    Serpins promote cancer cell survival and vascular co-option in brain metastasis. Valiente M, Obenauf AC, Jin X, Chen Q, Zhang XH, Lee DJ, Chaft JE, Kris MG, Huse JT, Brogi E, Massague J. Cell. 2014 Feb 27;156(5):1002-16. doi: 10.1016/j.cell.2014.01.040. 10.1016/j.cell.2014.01.040 PubMed 24581498
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