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pET14b-PITPalpha K61A
(Plasmid #58258)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58258 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 4671
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PITP alpha
  • Alt name
    phosphatidylinositol transfer protein alpha
  • Species
    H. sapiens (human)
  • Mutation
    K61A (abolishes PI binding)
  • Entrez Gene
    PITPNA (a.k.a. HEL-S-36, PI-TPalpha, PITPN, VIB1A)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET14b-PITPalpha K61A was a gift from Shamshad Cockcroft (Addgene plasmid # 58258 ; http://n2t.net/addgene:58258 ; RRID:Addgene_58258)

  • For your References section:

    Structure-function analysis of human [corrected] phosphatidylinositol transfer protein alpha bound to phosphatidylinositol. Tilley SJ, Skippen A, Murray-Rust J, Swigart PM, Stewart A, Morgan CP, Cockcroft S, McDonald NQ. Structure. 2004 Feb;12(2):317-26. 10.1016/j.str.2004.01.013 PubMed 14962392
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