Skip to main content
Addgene

pET14b-PITPalpha N90F
(Plasmid #58257)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58257 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 4671
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PITP alpha
  • Alt name
    phosphatidylinositol transfer protein alpha
  • Species
    H. sapiens (human)
  • Mutation
    N90F (abolishes PI binding)
  • Entrez Gene
    PITPNA (a.k.a. HEL-S-36, PI-TPalpha, PITPN, VIB1A)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET14b-PITPalpha N90F was a gift from Shamshad Cockcroft (Addgene plasmid # 58257 ; http://n2t.net/addgene:58257 ; RRID:Addgene_58257)

  • For your References section:

    Structure-function analysis of human [corrected] phosphatidylinositol transfer protein alpha bound to phosphatidylinositol. Tilley SJ, Skippen A, Murray-Rust J, Swigart PM, Stewart A, Morgan CP, Cockcroft S, McDonald NQ. Structure. 2004 Feb;12(2):317-26. 10.1016/j.str.2004.01.013 PubMed 14962392
Related items: