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Addgene

pET14b-PITPalpha
(Plasmid #58256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 4671
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PITP alpha
  • Alt name
    phosphatidylinositol transfer protein alpha
  • Species
    H. sapiens (human)
  • Entrez Gene
    PITPNA (a.k.a. HEL-S-36, PI-TPalpha, PITPN, VIB1A)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET14b-PITPalpha was a gift from Shamshad Cockcroft (Addgene plasmid # 58256 ; http://n2t.net/addgene:58256 ; RRID:Addgene_58256)

  • For your References section:

    The first 5 amino acids of the carboxyl terminus of phosphatidylinositol transfer protein (PITP) alpha play a critical role in inositol lipid signaling. Transfer activity of PITP is essential but not sufficient for restoration of phospholipase C signaling. Hara S, Swigart P, Jones D, Cockcroft S. J Biol Chem. 1997 Jun 6;272(23):14908-13. PubMed 9169461
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