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Addgene

pAdSh.PGK.Cas9
(Plasmid #58253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322-based pAdEasy backbone
  • Backbone size w/o insert (bp) 7499
  • Total vector size (bp) 11638
  • Vector type
    Mammalian Expression, Adenoviral, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human codon-optimized S. pyogenes Cas9
  • Alt name
    Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4139

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    RNA-guided human genome engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Feb 15;339(6121):823-6
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments


How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdSh.PGK.Cas9 was a gift from Manuel Goncalves (Addgene plasmid # 58253 ; http://n2t.net/addgene:58253 ; RRID:Addgene_58253)

  • For your References section:

    Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. 10.1038/srep05105 PubMed 24870050
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