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PurposeU6 promoter-driven single guide RNA complementary to the human AAVS1 locus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58252 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322-derived pAdEasy plasmid
- Backbone size w/o insert (bp) 6930
- Total vector size (bp) 7296
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Vector typeMammalian Expression, Adenoviral, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameU6.gRNAS1 cassette
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SpeciesSynthetic
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Insert Size (bp)366
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byRNA-guided human genome engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Feb 15;339(6121):823-6
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAdSh.U6.gRNAS1 was a gift from Manuel Goncalves (Addgene plasmid # 58252 ; http://n2t.net/addgene:58252 ; RRID:Addgene_58252) -
For your References section:
Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. 10.1038/srep05105 PubMed 24870050
Map uploaded by the depositor.
