pGLTR-X-GFP
(Plasmid
#58245)
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PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR-SIN-CSGW
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Backbone manufacturerAdrian Trasher, UCL, London, UK
- Total vector size (bp) 11300
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Vector typeMammalian Expression, Lentiviral, RNAi ; GATEWAY destionation vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameattR1-Cm(R)-CcdB-attR2
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Alt nameGATEWAY DEST cassette
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SpeciesGATEWAY DEST cassette
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Insert Size (bp)1580
- Promoter no
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site blunted EcoRI (unknown if destroyed)
- 3′ cloning site blunted EcoRI (unknown if destroyed)
- 5′ sequencing primer n.a.
- 3′ sequencing primer n.a. (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTetR-NLS-T2A-GFP
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Speciesrepressor/GFP fusion
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Insert Size (bp)700
- Promoter SFFV
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Tags
/ Fusion Proteins
- TetR
- NLS
- T2A
- GFP
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n.a.
- 3′ sequencing primer n.a. (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGATEWAY DEST-cassette (Invitrogen)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGLTR-X-GFP was a gift from Stephan Geley (Addgene plasmid # 58245 ; http://n2t.net/addgene:58245 ; RRID:Addgene_58245) -
For your References section:
Development of a Multipurpose GATEWAY-Based Lentiviral Tetracycline-Regulated Conditional RNAi System (GLTR). Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S. PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014. 10.1371/journal.pone.0097764 PubMed 24841113