pGLTR-S-PURO
(Plasmid #58244)

• Purpose: GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance
• Depositing Lab: Stephan Geley
• Publication: Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014.

Backbone

• Vector backbone: pHR-SIN-CSGW
• Backbone manufacturer: Adrian Trasher, UCL, London, UK
• Total vector size (bp): 11300
• Vector type: Mammalian Expression, Lentiviral, RNAi ; GATEWAY destination vector
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: attR1-Cm(R)-CcdB-attR2
• Alt name: GATEWAY DEST cassette
• Species: GATEWAY DEST cassette
• Insert Size (bp): 1580
• Promoter: no

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: blunted EcoRI (unknown if destroyed)
• 3′ cloning site: blunted EcoRI (unknown if destroyed)
• 5′ sequencing primer: n.a.
• 3′ sequencing primer: n.a.

Gene/Insert 2

• Gene/Insert name: Puro(R)
• Alt name: puromycin N-acetyl transferase (pac) gene
• Species: Streptomyces alboniger
• Insert Size (bp): 606
• GenBank ID: M25346.1
• Promoter: SFFV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n.a.
• 3′ sequencing primer: n.a.

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GATEWAY DEST-cassette (Invitrogen)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.