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Addgene

pMIR-DsRed-IRES-His-Halo-Tev-Keap1
(Plasmid #58240)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIR
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 9330
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kelch like ECH associated protein 1
  • Alt name
    Keap1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2859
  • GenBank ID
    NM_203500.1 NM_012289.3
  • Entrez Gene
    KEAP1 (a.k.a. INrf2, KLHL19)
  • Promoter CMV
  • Tag / Fusion Protein
    • His-Halo-Tev (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TGGCTCTCCTCAAGCGTATT
  • 3′ sequencing primer CAGGAAACAGCTATGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Yen, H-C.S., Xu, Q., Chou, D.M., Zhao, Z., and Elledge, S.J. (2008). Global protein stability
profiling in mammalian cells. Science 322, 918−923

Aye, Y.; Brignole, E. J.; Long, M. J. C.; Chitturulu, J.; Drennan, C. L.; Asturias, F. J.;
Stubbe J. Chem. Biol. 2012, 19, 799-805

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-DsRed-IRES-His-Halo-Tev-Keap1 was a gift from Yimon Aye (Addgene plasmid # 58240 ; http://n2t.net/addgene:58240 ; RRID:Addgene_58240)

  • For your References section:

    Temporally controlled targeting of 4-hydroxynonenal to specific proteins in living cells. Fang X, Fu Y, Long MJ, Haegele JA, Ge EJ, Parvez S, Aye Y. J Am Chem Soc. 2013 Oct 2;135(39):14496-9. doi: 10.1021/ja405400k. Epub 2013 Sep 18. 10.1021/ja405400k PubMed 24015839
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