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Addgene

PLEXIN A3
(Plasmid #58238)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS KS+
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plexin A3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    800
  • Entrez Gene
    Plxna3 (a.k.a. PlexA3, Plxa3, Plxn3, Plxn4, SEX, mKIAA4078)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA from expressed sequence tag (EST) IMAGE clones (Invitrogen), clone ID 401253.
For antisense RNA transcription, use XhoI and T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLEXIN A3 was a gift from Christiana Ruhrberg (Addgene plasmid # 58238 ; http://n2t.net/addgene:58238 ; RRID:Addgene_58238)

  • For your References section:

    Plexin A3 and plexin A4 convey semaphorin signals during facial nerve development. Schwarz Q, Waimey KE, Golding M, Takamatsu H, Kumanogoh A, Fujisawa H, Cheng HJ, Ruhrberg C. Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10.1016/j.ydbio.2008.08.020. Epub 2008 Sep 3. 10.1016/j.ydbio.2008.08.020 PubMed 18804103
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