pFGL822-8
(Plasmid #58226)

• Purpose: For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
• Depositing Lab: Naweed Naqvi
• Publication: Sweigard et al Fungal Genetics Reports, 1997. Vol. 44, Article 19

Backbone

• Vector backbone: pFGL815
• Modifications to backbone: Bialaphos resistance (Bar) cassette (0.9kb) has been cloned into pFGL815 (Addgene 52322) between Pst1 and Sal1 sites.
• Vector type: Bacterial Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phosphinothricin acetyl transferase
• Alt name: bialaphos resistance (Bar) gene
• Species: Streptomyces sp.
• Insert Size (bp): 945
• GenBank ID: AF013602
• Promoter: Aspergillus nidulans trpC promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GATCCTCGAGTGATATTGAAGGAGCACTTTTTGGGC
• 3′ sequencing primer: CTGGTCGACCTAAATCTCGGTGACGGGCAGGACCGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCB1530 from FGSC (Fungal Genetics Stock Center).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53