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Addgene

pFGL821
(Plasmid #58223)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGL815
  • Modifications to backbone
    Hygromycin B resistance (hptII) cassette (1.4kb) has been cloned into pFGL815 (Addgene 52322) between Xba1 and Sal1 sites.
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hygromycin B phosphotransferase
  • Alt name
    hptII
  • Species
    Escherichia coli
  • Insert Size (bp)
    1422
  • GenBank ID
  • Promoter Aspergillus nidulans trpC promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GATCCTCGAGTGATATTGAAGGAGCACTTTTTGGGC
  • 3′ sequencing primer GACGTCGACTGGTTCCGGTCGGCATCTACTCTATT
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCB1003 from FGSC (Fungal Genetics Stock Center).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGL821 was a gift from Naweed Naqvi (Addgene plasmid # 58223 ; http://n2t.net/addgene:58223 ; RRID:Addgene_58223)

  • For your References section:

    A series of vectors for fungal transformation. Sweigard JA, Chumley F, Carroll A, Farrall L, Valent B. Fungal Genetics Reports, 1997. Vol. 44, Article 19 10.4148/1941-4765.1287
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