pFGL820
(Plasmid #58221)

• Purpose: For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.
• Depositing Lab: Naweed Naqvi
• Publication: Sweigard et al Fungal Genetics Reports, 1997. Vol. 44, Article 19

Backbone

• Vector backbone: pFGL815
• Modifications to backbone: Sulfonylurea resistance (SUR) cassette (2.8kb)has been cloned into pFGL815 (Addgene 52322) between Xba1 and Sal1 sites.
• Vector type: Bacterial Expression
• Selectable markers: Sulfonylurea (chlorimuron ethyl)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ILV2
• Alt name: SUR
• Species: Magnaporthe grisea
• Insert Size (bp): 2830
• GenBank ID: AF013601
• Promoter: ILV2 native promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CACCTCGAGGTGCCAACGCCACAGTGCCCCACATCT
• 3′ sequencing primer: CGACGTCGACATGCAATTCCCGTGCAATAATCAATTCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCB1550 from FGSC (Fungal Genetics Stock Center).
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53

Please note that the actual sequence of the SUR gene in this plasmid differs from the Genbank reference sequence AF013601 by a G120V mutation in the ILV2 locus. G120V does not contribute to the sulfonyl urea resistance per se.