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Purpose(Empty Backbone) Localization: Cytosolic FRET, Excitation: , Emission:
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- mNeonGreen
- mRuby2
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mNeonGreen
- mRuby2
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FRET Indicator - Live and Fixed Cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mNeonGreen-mRuby2-FRET-10 was a gift from Michael Davidson (Addgene plasmid # 58179 ; http://n2t.net/addgene:58179 ; RRID:Addgene_58179) -
For your References section:
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Shaner NC, Lambert GG, Chammas A, Ni Y, Cranfill PJ, Baird MA, Sell BR, Allen JR, Day RN, Israelsson M, Davidson MW, Wang J. Nat Methods. 2013 May;10(5):407-9. doi: 10.1038/nmeth.2413. Epub 2013 Mar 24. 10.1038/nmeth.2413 PubMed 23524392