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Addgene

tdTomato-CENPB-N-22
(Plasmid #58081)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    tdTomato-N1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENPB
  • Alt name
    NM_001810.5
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-169 of CENPB (DNA Binding Domain)
  • Entrez Gene
    CENPB
  • Promoter CMV
  • Tag / Fusion Protein
    • tdTomato (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 554; Emission = 581

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tdTomato-CENPB-N-22 was a gift from Michael Davidson (Addgene plasmid # 58081 ; http://n2t.net/addgene:58081 ; RRID:Addgene_58081)

  • For your References section:

    Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens. Gao L, Shao L, Higgins CD, Poulton JS, Peifer M, Davidson MW, Wu X, Goldstein B, Betzig E. Cell. 2012 Dec 7;151(6):1370-85. doi: 10.1016/j.cell.2012.10.008. 10.1016/j.cell.2012.10.008 PubMed 23217717
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