mTagRFP-Membrane-1
(Plasmid #57992)

• Purpose: (Empty Backbone) Localization: Plasma Membrane, Excitation: 555, Emission: 584
• Depositing Lab: Michael Davidson
• Publication: Davidson Orange Fluorescent Proteins (unpublished)

Backbone

• Vector backbone: mTagRFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)
• Tags / Fusion Proteins:
  • N-terminal 20 Aas MLS from Neuromodulin (C terminal on backbone)
  • mTagRFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • N-terminal 20 Aas MLS from Neuromodulin (C terminal on backbone)
  • mTagRFP (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • mTagRFP-Membrane-1 Data Supplement
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

N-terminal 20 AAs from Neuromodulin. Excitation = 555; Emission = 584