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mOrange2-Keratin-17
(Plasmid #57965)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 57965 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mOrange2
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Keratin
  • Alt name
    NM_199187.1
  • Species
    H. sapiens (human)
  • Mutation
    V282L
  • Entrez Gene
    KRT18 (a.k.a. CK-18, CYK18, K18)
  • Promoter CMV
  • Tag / Fusion Protein
    • mOrange2

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 549; Emission = 565

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mOrange2-Keratin-17 was a gift from Michael Davidson (Addgene plasmid # 57965 ; http://n2t.net/addgene:57965 ; RRID:Addgene_57965)

  • For your References section:

    Improving the photostability of bright monomeric orange and red fluorescent proteins. Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY. Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4. 10.1038/nmeth.1209 PubMed 18454154
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