mOrange2-CytERM-N-17
(Plasmid
#57952)
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PurposeLocalization: Endoplasmic Reticulum, Excitation: 549, Emission: 565
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 57952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemOrange2-N1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCytERM (AA1-29)
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Alt nameCytochrome p450
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Alt nameNM_001171120.1
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MutationOnly first 29 AA of CytERM with mutations V8G, H24Y present in this plasmid
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GenBank ID
- Promoter CMV
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Tag
/ Fusion Protein
- mOrange2 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cytochrome p450 . Excitation = 549; Emission = 565
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mOrange2-CytERM-N-17 was a gift from Michael Davidson (Addgene plasmid # 57952 ; http://n2t.net/addgene:57952 ; RRID:Addgene_57952)
