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Addgene

mKO-Vinculin-23
(Plasmid #57852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 57852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mKO
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Alt name
    NM_003373.3
  • Species
    H. sapiens (human)
  • Entrez Gene
    VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
  • Promoter CMV
  • Tag / Fusion Protein
    • mKO

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 548; Emission = 561

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mKO-Vinculin-23 was a gift from Michael Davidson (Addgene plasmid # 57852 ; http://n2t.net/addgene:57852 ; RRID:Addgene_57852)

  • For your References section:

    Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, Davidson MW, Fetter RD, Hess HF. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 10.1073/pnas.0813131106. Epub 2009 Feb 6. 10.1073/pnas.0813131106 PubMed 19202073
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