mKO-Fibrillarin-7
(Plasmid
#57842)
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PurposeLocalization: Nuclear - Nucleolus, Excitation: 548, Emission: 561
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 57842 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemKO
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFibrillarin
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Alt nameNM_001436.3
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SpeciesH. sapiens (human)
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MutationR36Q, S66F
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Entrez GeneFBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
- Promoter CMV
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Tag
/ Fusion Protein
- mKO (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 548; Emission = 561
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mKO-Fibrillarin-7 was a gift from Michael Davidson (Addgene plasmid # 57842 ; http://n2t.net/addgene:57842 ; RRID:Addgene_57842) -
For your References section:
Fluorescent protein tracking and detection: fluorescent protein structure and color variants. Rizzo MA, Davidson MW, Piston DW. Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63. 10.1101/pdb.top63 PubMed 20150100
