pLKO5.sgRNA.EFS.PAC
(Plasmid #57825)

• Purpose: Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven
• Depositing Lab: Benjamin Ebert
• Publication: Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.

Backbone

• Vector backbone: pLKO.005
• Total vector size (bp): 7295
• Vector type: Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: sgRNA
• Insert Size (bp): 75

Gene/Insert 2

• Gene/Insert name: EFS
• Alt name: short EF1-alpha promoter
• Insert Size (bp): 250

Gene/Insert 3

• Gene/Insert name: PAC
• Alt name: Puromycin resistance gene
• Species: Synthetic
• Insert Size (bp): 650

Resource Information

• Supplemental Documents:
  • Target site cloning protocol
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use BsmBI sites for sgRNA cloning. Note that this plasmid does NOT contain the 1.9kb stuffer from the pLKO.005 vector backbone.